Comparative effect of bovine buttermilk, whey, and lactoferrin on the innate immunity receptors and oxidative status of intestinal epithelial cells

Milk contains bioactive molecules with important functions as defensive proteins; among them are the whey protein lactoferrin and proteins of milk fat globule membrane (MFGM) present in buttermilk. The aim this study has been to investigate effects lactoferrin, whey, buttermilk modulators intestinal innate immunity oxidative stress on epithelial cells, evaluate its potential use for development functional foods. mRNA expression levels immune system Toll-like receptors (TLR2, TLR4, TLR9), lipid peroxidation (malondialdehyde + 4-hydroxyalkenals) carbonyl were analyzed enterocyte-like Caco-2/TC7 cells treated 24 h different concentrations or None substances caused damage; however, significantly decreased peroxidation. Furthermore, both reduced induced by lipopolysaccharide. With respect TLR receptors, specifically altered TLR2, TLR9 a strong decrease TLR4. These results suggest that potentially interesting ingredients foods because they seem modulate inflammatory response activation TLRs.